Status : Taken down
Personal Name Ayala, Mary Grace B.
Resource Title Methicillin - resistant Staphylococcus aureus carriage in a residential care institution for senior citizens in Quezon City, Philippines
Date Issued December 2016
Abstract Antimicrobial drug resistance in Staphylococcus aureus is a serious burden to public health. The occurrence of multidrug-resistant (MDR) strains further complicates the treatment of infections. While antimicrobial resistance in hospitals is monitored by government agencies, studies on the prevalence of MDR S. aureus in other healthcare facilities are limited. This study aimed to determine the prevalence, antimicrobial susceptibility patterns, and risk factors associated with S. aureus, methicillin-resistant S. aureus (MRSA), and MDR S. aureus carriage in the Golden Reception and Action Center for the Elderly and other Special Cases (GRACES). It also aimed to identify the types of staphylococcal cassette chromosome mec (SCCmec) in the MRSA isolates. Sixty-six nasal swabs were collected from asymptomatic elderly residents. Staphylococcus aureus was identified by biochemical tests and PCR amplification of the nuc gene. Antimicrobial susceptibility assays and PCR amplification of mecA gene were performed to determine methicillin resistance. Results show that the prevalence for S. aureus was 37.9% among the residents. Eleven of the 25 S. aureus isolates (44%) were MRSA, corresponding to a prevalence of 16.7% among the residents. SCCmec typing showed that all of the MRSA isolates carried the SCCmec type IV. MDR, which is the resistance of the organism to at least one antimicrobial agent in three or more categories (Magiorakos et al., 2012), was
observed in 14 S. aureus isolates (56%) which correspond to 21.2% prevalence among the residents. Of these, 10 were MDR MRSA and four were MDR methicillin- susceptible S. aureus (MDR MSSA). Carriage of S. aureus (p=0.048; OR=2.86; 95% CI=0.99-8.26) and MRSA (p=0.033; OR=4.67; 95% CI=1.19-18.26) was significantly associated with history of pulmonary tuberculosis, but no association (p>0.05) was observed in MSSA. Significant association between history of pulmonary tuberculosis and carriage of MDR S. aureus (p=0.010; OR=5.40; 95% CI=1.53-19.05) and MDR MRSA (p=0.012; OR=6.38; 95% CI=1.46-27.91) was also observed, but no association (p>0.05) in MDR MSSA. Results show that asymptomatic elderly residents may carry S. aureus and MDR S. aureus including MRSA. Routine surveillance and mitigation controls should, therefore, be implemented to prevent possible outbreaks of S. aureus infections in a residential care facility.
Degree Course Master of Science in Microbiology
Language English
Keyword Staphylococcus aureaus, multi-drug resistant (MDR)
Material Type Thesis/Dissertation