Status : Taken down
Personal Name Delfin, Jay C.
Resource Title Antioxidative response, total phenols and cadmium distribution in salicylic acid-treated Euphorbia hirta L.
Date Issued May 2016
Abstract The effects of exogenous salicylic acid (SA) on the antioxidative response of cadmium-exposed Euphorbia hirta L. and on the distribution of Cd within the plant were investigated. Mature E. hirta plants were transferred to hydroponics, sprayed with 500 µM SA on their leaves, and exposed to 5 ppm CdCl2 for 3 days. The total chlorophyll concentrations of SA- and Cd-treated plants were significantly reduced compared with that of the control, but no significant reduction was observed in SA+Cd-treated plants. Lipid peroxidation was highest in Cd-treated plants but was slightly reduced in SA- and SA+Cd- treated plants. The levels of H2O2 significantly increased in plants treated with SA or Cd, but the increase was insignificant when SA was applied prior to Cd exposure. Catalase activity was significantly inhibited in plants treated with either SA or Cd treatment or both, whereas, superoxide dismutase activity was enhanced by either or both treatments. SA or Cd treatment significantly increased the total phenols in the plant’s shoot, which was consistent with the observed enhanced phenylalanine ammonia lyase activity in the treated plants. Endogenous SA level was higher in Cd-treated plants relative to that in the control, but was slightly lower whenever exogenous SA was applied. Cd2+ content was greater in the roots compared to the stems and leaves in both Cd- and SA+Cd-treated plants, and was significantly higher in the leaves of SA+Cd-treated plants than in the leaves of Cd-treated plants. The results suggest that SA treatment alleviated the Cd-induced oxidative damage in E. hirta by promoting the production of phytophenols and modifying the antioxidant enzyme activities.
Degree Course Master of Science in Biology
Language English
Keyword antioxidants, cadmium, catalase, chlorophyll, Euphorbia hirta, lipid peroxidation, salicylic acid, phenols, superoxide dismutase
Material Type Thesis/Dissertation