Status : Verified
| Personal Name | Delos Reyes, Nikko B. |
|---|---|
| Resource Title | 3D printed and molecularly imprinted acrylic polymer for oxytetracycline sensing using handheld NIR spectroscopy |
| Date Issued | 26 June 2020 |
| Abstract | Environmental monitoring of antibiotics, such as oxytetracycline (OTC), in aqueous bodies, is necessary to minimize the risk of transmission of antibiotic-resistant bacteria to humans, the risk of accelerating evolution of antibiotic resistance, and address the risks antibiotics aquatic ecosystem. Moreover, commonly accepted methods of environmental monitoring of OTC were listed with their drawbacks: (a) chromatographic: slow, laborious sample pretreatment, expensive and requires skilled operator; (b) biosensor based (immunoassays): chemically and thermally unstable, and (c) laboratory NIR: bulky equipment and low sensitivity. Thus, this study aimed to develop a rapid, on-site, handheld, and sensitive probing method for OTC presence detection and determination in an aqueous medium. This study investigated the feasibility of using the following media for detection of OTC presence and determination of OTC concentration: aqueous, 3D printed adsorbent, and molecularly imprinted polymer (MIP) – coated 3D printed adsorbent. Moreover, machine learning models were developed for each medium and corresponding application using absorbance spectral data from a handheld NIR spectrometer. The results of the study showed almost perfect classification (only 1 misclassification of 109 samples) for the prediction set for OTC detection presence of concentration 50 ppm – 300 ppm in an aqueous medium. Finally, tuned K Nearest Neighbor model yielded reliable concentration determination, with rC = 1.00 and RMSE = 0.00 for 50 ppm – 300 ppm OTC in aqueous medium using 3D printed substrates and MIP-coated 3D printed substrates. |
| Degree Course | MS Chemical Engineering |
| Language | English |
| Keyword | molecular imprinting; 3D printing; oxytetracycline; NIR spectroscopy |
| Material Type | Thesis/Dissertation |
Preliminary Pages
412.39 Kb
Category : F - Regular work, i.e., it has no patentable invention or creation, the author does not wish for personal publication, there is no confidential information.
Access Permission : Open Access