Status : Verified
| Personal Name | Esporlas, Juan Miguel V. |
|---|---|
| Resource Title | In vitro release and kinetic modeling of Citropin 1.1 antimicrobial peptide-loaded chitosan hydrogels prepared via pH-induced gelation |
| Date Issued | 01 July 2024 |
| Abstract | The decline in antibiotic discovery and increasing restrictiveness of antibiotics continue to strengthen antimicrobial resistance (AMR) as a potentially significant global threat. Of the many emerging solutions, antimicrobial peptides (AMP) are known for their efficiency in treating multi- drug resistant (MDR) organisms. However, drawbacks on its stability, bioavailability, and unwanted macromolecule interactions hamper its clinical use. Considering these limitations, the development of better drug delivery systems is needed to increase the stability, efficacy, and pharmacokinetics of AMPs. Chitosan-based hydrogels (CSH), known for their excellent therapeutic agent carrier performance, were synthesized via the pH-induced gelation method. Citropin 1.1 peptide (CIT) and varying concentrations of Doxycycline (DOX), Vancomycin (VCY), and Methylene blue dye (MB) were encapsulated into the CSH. Doxycycline was used to compare low molecular weight drug release behavior with AMP, while Vancomycin was used to compare high molecular weight drug release behavior with AMP. On the other hand, MB dye was used to compare an easily detectable agent’s release behavior with AMP. Additionally, the release profiles of the loaded CSH in phosphate-buffered saline (PBS) were investigated via a UV-VIS spectrophotometer. In vitro release data showed that the DOX-CSH, VCY-CSH, and MB-CSH followed a similar pattern: initial burst release, followed by a sustained release, and eventually reaching an equilibrium state, while CIT-CSH observed a different pattern of release: sustained release, followed by a burst release and eventually reaching equilibrium state. The highest overall efficiency reported for DOX-CSH, VCY-CSH, MB-CSH, and CIT-CSH are 16.86 ± 0.75%, 6.02 ± 0.26%, 6.77 ± 1.18% and 55.11 ± 4.71%, respectively. The release data of loaded hydrogels were modeled with Korsmeyer-Peppas (KP), Higuchi (H), and First-Order (FO) kinetic models. The best-fit model for CIT-CSH was observed w |
| Degree Course | MS Chemical Engineering |
| Language | English |
| Keyword | antimicrobial resistance; drug delivery systems; chitosan hydrogels; Citropin 1.1; Doxycycline; Vancomycin; Methylene blue dye |
| Material Type | Thesis/Dissertation |
Preliminary Pages
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Category : I - Has patentable or registrable invention of creation.
Access Permission : Limited Access