Status : Verified
| Personal Name | Rosil, Shaina Mae C. |
|---|---|
| Resource Title | Evaluating the Degradation Potential of a Novel Alkaliphilic Bacterial Isolate into the Distillate Derived Strong Alkaline Wastewater from PGME Processes using Batch Experiments |
| Date Issued | 10 June 2025 |
| Abstract | The rising demand for effective wastewater treatment solutions and technologies has become vital due to increasing industrial activities and the associated environmental and public health risks posed by industrial effluents. Propylene Glycol Methyl Ether (PGME), an organic solvent widely used in semiconductor manufacturing, ink solvent, paint production as a coating agent, cleaning agent, leather dyeing and pesticide raw material contributes to high-pH wastewater, and high chemical oxygen demand (COD) presenting challenges to treatment methods. This study investigates the degradation potential of an alkaliphilic bacterial isolate, initially labeled as SBR-02 in the reduction of chemical oxygen demand (COD) of distillate-derived strong alkaline wastewater (D-DSAW) from PGME processes (pH = 13.9) through batch experiments. A response surface methodology (RSM) using the Box-Behnken Design (BBD) in Design Expert 13 software was employed to examine the effects of initial COD concentration, initial pH, and inoculum size on biodegradation efficiency. The results indicated that SBR-02 effectively reduced COD, achieving an optimal removal efficiency of 68.33% observed under conditions of 3000 mg/L initial COD concentration, pH 10 and 25% v/v inoculum size. Statistical analysis through ANOVA confirmed the significant influence of these parameters on the degradation performance with initial pH and inoculum size showing the most significant interaction effects (F-value= 72.57, p-value = 0.0001). 16S rRNA gene sequencing identified the bacterial strain as closely related to Halalkalibacter krulwichiae strain AM31D with 95.08% identity and 100% query cover, a member of the Bacillaceae family known for its enzymatic degradation capabilities. The findings suggest that alkaliphilic bacteria hold promise for industrial wastewater bioremediation, providing insights into microbial-based treatment approaches for highly alkaline waste streams. |
| Degree Course | Master of Science in Environmental Engineering |
| Language | English |
| Keyword | Alkaliphilic bacteria, alkaline wastewater treatment, bioremediation, PGME wastewater |
| Material Type | Thesis/Dissertation |
Preliminary Pages
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Category : P - Author wishes to publish the work personally.
Access Permission : Limited Access