This study developed a teacher preparation framework based on empirical evidence of the effects of transdisciplinary biology instruction with Generative Al on pre-service teachers’ Technology, Learner, Pedagogy, Content, and Context Knowledge (TLPACK), digital citizenship, and cognitive offloading. It also looked into the moderating effect of GenAl literacy on Biology instruction with Generative Al integration, and the interactions among TLPACK, digital citizenship, and cognitive offloading of pre-service teachers.
A convergent parallel mixed methods research design was utilized, where the quantitative strand was a quasi-experiment involving a three-group repeated measures factorial configuration, while the qualitative strand was a multiple case study design. A total of 92 pre-service teachers (PSTs) from three comparable intact sections that were enrolled in a science education course in a state university were involved in the study. After random determination, one group was exposed to the intervention combining Universal Design for Learning, Generative Al, and Transdisciplinary Biology Instruction or UGTBI (n = 29). Another group was exposed to the intervention combining Generative Al and Transdisciplinary Biology Instruction or GTBI (n = 30), while the third group was considered as the comparison group (CBI, n = 33). Data on participants’ TLPACK, digital citizenship, and cognitive offloading were collected using researcher-developed instruments with
acceptable reliability.
Results of the one-way MANOVA, converging completely with the qualitative findings, showed that both UGTBI and GTBI were effective in improving pre-service teachers’ TLPACK and digital citizenship. On the other hand, results of the aligned rank transform (ART) ANOVA revealed increase in the participants’ cognitive offloading across seven learning cycles in favor of the intervention groups (UGTBI and GTBI). Structural Equation Modeling (SEM) revealed the following significant interactions among pre-service teachers’ outcomes in a biology class: (a) TLPACK directly influences digital citizenship; (b)
digital citizenship directly affects cognitive offloading; and (c) TLPACK does not directly influence cognitive offloading unless mediated by digital citizenship. Lastly, another set of SEM analyses established that Generative Al literacy does not moderate the effects of Transdisciplinary Biology Instruction on pre-service teachers’ TLPACK, digital citizenship,
and cognitive offloading.
Based on the results, a transdisciplinary STEM teacher preparation framework was conceptualized, which emphasized the progression of pre-service teachers from theoretical learning to in-service practice. The paradigm was grounded on the TLPACK framework, with specific focus on acquisition, application, and reflection, which are integrated into three progressive phases: pre-service didactics, pre-service practicum, and in-service training. The design ensures that pre-service teachers not only acquire requisite knowledge but also apply it effectively in various instructional contexts.
Moreover, the results of this study provide critical insights for the advancement of Biology Education, particularly on the aspects of strengthening transdisciplinarity, integrating generative Al, implementing Universal Design for Learning, and understanding the dynamics among TLPACK, digital citizenship, and cognitive offloading. These aim to enhance pre- service teachers’ capacity to design inclusive, ethical, and adaptive instruction, which is crucial not only for future-ready STEM education, but for elevating the overall quality, equity, and responsiveness of teaching in the 21st-century educational landscape. It is recommended that pre-service education programs implement a structured transdisciplinary teacher preparation framework that integrates use of Generative Al, TLPACK, digital citizenship, and cognitive offloading as part of a developmental continuum.